CHANGES IN SPERM MOTILITY AND ITS REGULATION BY PROTEIN KINASE C AND TYROSINE KINASE IN SPERM CAPACITATION WITH HYALURONIC ACID OR HEPARIN

FERNÁNDEZ S; CÓRDOBA M

reunion virtual

Congreso; IV Reunión Conjunta de Sociedades de Biología de la República Argentina ?Nuevas evidencias y cambios de paradigmas en ciencias biológicas; 2020

Sociedades de Biología de la República Argentina

CHANGES IN SPERM MOTILITY AND ITS REGULATION BY PROTEIN KINASE C AND TYROSINE KINASE IN SPERM CAPACITATION WITH HYALURONIC ACID OR HEPARINFernández S, Córdoba MInstituto de Investigación y Tecnología en Reproducción Animal (INITRA, UBA),Unidad ejecutora de Investigaciones en Producción Animal (INPA, UBA-CONICET),Cátedra de Química Biológica, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina. mcordoba@fvet.uba.arDuring capacitation, sperm motility increases to allow sperm to interact with the zona pellucida and trigger acrosomal exocytosis. Motility could be enhanced by the use of hyaluronic acid or heparin, so our aim was to evaluate it and to study the participation of protein kinase C and tyrosine kinase in its regulation in cryopreserved bovine spermatozoa. Hyaluronic acid and heparin are glycosaminoglycans present in the bovine female genital tract which induce capacitation in this species during in vitro conditions. GF-109203X and genistein were used as protein kinase C and tyrosine kinase inhibitors, respectively. Capacitation was evaluated by the chlorotetracycline epifluorescent technique and viability by trypan blue staining. Sperm motility was analyzed by Computer Assisted Sperm Analysis (ISAS). Data were analyzed by ANOVA and Tukey test (P