Endogenous lipid and aminoacid metabolism during bovine oocyte maturation in a defined media without oxidative substrates

MARTINEZ SANTIAGO; CETICA PABLO; GAGNETEN PAULA; GUTNISKY CYNTHIA; BREININGER ELIZABETH

Mar del Plata

Congreso; Reunión de Sociedades Biocientíficas (SAIC-SAFE-SAB-SAP); 2019

SAIC, SAFE, SAB, SAP

ENDOGENOUS LIPID AND AMINOACID METABOLISM DURING BOVINE OOCYTE MATURATION IN A DEFINED MEDIA WITHOUT OXIDATIVE SUBSTRATES GAGNETEN P.1, MARTINEZ S.1, BREININGER E.1,2, GUTNISKY C.1,2, CETICA P.1,21UBA, Facultad de Cs Veterinarias, INITRA, Química Biológica; 2UBA- CONICET, INPA.Metabolic studies carried out in cumulus oocytes-complexes (COCs) usually refer to carbohydrates as they are the main substrates in the culture media. There is little information about endogen lipids (EL) and aminoacids (AA) as oxidative substrates. The aim of this work was to evaluate the nuclear maturation in a defined media lacking other oxidative substrates. Maturation was performed in a media without oxidative substrates, SOFm (without pyruvate and lactate) supplemented with FSH, LH, EGF, insulin and PVA, under mineral oil at 39ºC in humidified atmosphere for 22h. For the EL evaluation oocytes were randomly divided in 4 groups: positive control1 (+C1): SOF+glucose 5,5mM, positive control2 (+C2): SOF+L-Carnitine (β-oxidation fatty acid stimulator), negative control (-C): SOF+etomoxir (β-oxidation fatty acid inhibitor) and treatment (T): without any supplementation and for the AA evaluation: positive control (+C): SOF+glucose+AA, treatment (T): SOF+AA, negative control1 (-C1): SOF+ sodium salicylate (inhibitor of glutamate dehydrogenase) and negative control2 (-C2): without any supplementation. The proportion of matured oocyte was evaluated by the presence of metaphase II after staining with Hoechst 33342. For the EL evaluation, the presence of carnitine (+C2) increased the nuclear maturation rate respect to the -C and the T groups (P