The antagonic behavior of GPIHBP1 between EAT and circulation does not reflect lipolytic enzymes levels in the tissue and serum from coronary patients

NAGASAWA, TAKUMI; BALDI, JULIO; SCHREIER, LAURA; BARCHUK, MAGALÍ; LÓPEZ, GRACIELA; RUBIO, MIGUEL; BERG, GABRIELA; MURAKAMI, MASAMI; MIKSZTOWICZ, VERÓNICA; NAKAJIMA, KATSUYUKI

CLINICA CHIMICA ACTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2020 vol. 510 p. 423 - 429

0009-8981

triglyceride rich lipoproteins are proposed to contribute to CAD risk; its concentrations would be partly determined by lipoprotein lipase (LPL) and endothelial lipase (EL). Epicardial adipose tissue (EAT), a visceral ATsurrounding myocardium and coronary arteries, emerged as an important actor in CAD; the increase in itsvolume could be a consequence of LPL and EL. Circulating enzymes levels would be conditioned by local tissuefactors. Our aim was to evaluate LPL, EL and their regulators levels in serum and EAT from CAD patients,searching for possible parallelisms and their role in the lipoprotein profile.Methods: In serum, EAT and subcutaneous AT (SAT) from patients undergoing coronary artery bypass graft(CABG, n = 25) or valve replacement (No CABG, n = 25), LPL, EL and glycosylphosphatidylinositol-anchoredhigh density lipoprotein-binding protein-1 (GPIHBP1) expression were evaluated. Besides, Apoprotein (Apo)CII,CIII and AV were determined in serum, along with lipoprotein profile.Results: Insulin-resistance markers were higher in CABG (p < 0.05). Serum LPL levels were decreased(p = 0.045), while EL levels increased (p < 0.001) in CABG, without differences in EAT or SAT. CirculatingGPIHBP1 concentrations were decreased in CABG (p = 0.047), while EAT GPIHBP1 expression was increased(p < 0.001). ApoCII and ApoAV concentrations were higher in CABG (p = 0.016 and p = 0.047, respectively),without differences in ApoCIII concentrations between groups.Conclusions: In EAT, LPL and EL protein levels were not changed in CAD, although GPIHBP1 protein levels werehigher. EAT would be a minor contributor to the circulating levels of the enzymes.