Nanoscopy in the Neurosciences

BARRANTES, FRANCISCO J.

MICROSCOPY & MICROANALYSIS

CAMBRIDGE UNIV PRESS

Año: 2020 vol. 26 p. 127 - 128

1431-9276

Superresolution optical microscopy (nanoscopy) is increasingly being applied in the Neurosciences [1-3]. This is because nanoscopy in its various implementations covers a wide range of possibilities in the space-time palette. Among the variants of superresolution microscopy, two alternative approaches stand out: targeted strategies, like STED (Stimulated Emission Depletion superresolution microscopy) and the stochastic methods, like PALM (Photo-Activated Localization Microscopy) and STORM (Stochastic Optical Reconstruction Microscopy). The former is a ?what-you-see-is-what-you-get? type of direct method, in which the fixed specimen is scanned to yield a diffraction-unlimited image. The technique is usually implemented together with confocal scans of the same specimen, thereby providing a direct comparison of the improved resolution accomplished with the nanoscopic imaging.