PROSTAGLANDIN E2 (PGE2) ENHANCES EFFEROCYTOSIS IN HUMAN MONOCYTE-DERIVED MACROPHAGES

A. PALETTA; A. CEBALLOS; F. DI DIEGO GARCÍA; F. REMES LENICOV; G. CABRERIZO; J. GEFFNER

Congreso; Reunión de Sociedades de Biociencias 2020; 2020

SAIC - SAFICI -SAI

Phagocytosis of apoptotic neutrophils by macrophages is one key feature in the resolution of inflammation. Recent studies have shown that prostaglandin E2 (PGE2), an immune mediator with recognized inflammatory properties, is also required for the initiation of the resolution process. Here we investigated the effect of PGE2 on neutrophil phagocytosis by macrophages.Human monocytes were obtained from blood buffy coats and differentiated to macrophages by culture with M-CSF (50 ng/ml) for 7 days. Macrophages were treated or not (control) with PGE2 (10-6 M) during the last 24 and 48 hours of culture. Polymorphonuclear leukocytes (PMNs) were purified and cultured in RPMI medium containing 5% fetal bovine serum overnight to induce apoptosis (>75% annexin V positive). Apoptotic PMNs were stained with CFSE, added to macrophages for 1 hour and washed. Macrophages were then collected and efferocytosis was measured by flow cytometry. We observed that treatment with PGE2 promoted macrophage capacity to engulf apoptotic neutrophils, as demonstrated by an average 15% increase of phagocytic macrophages (treated vs control, n=10, p