Antiretroviral treatment did not restore functionality of cervical mucosal cells for Th17-related cytokines altered after HIV infection.

MARÍA PÍA HOLGADO; DIEGO ZURITA; NATALIA LAUFER; OMAR SUED; JULIANA FALIVENE; ANGELES NICO; PÉREZ HECTOR MIGUEL; GABRIELA TURK; MARIA PAULA CARUSO; JIMENA SALIDO; VALERIA FINK; PEDRO CAHN; MARÍA MAGDALENA GHERARDI

Amsterdam

Congreso; 22nd International AIDS Conference; 2018

IAS

ACEPTADO PARA SU PRESENTACIÓN EN FORMATO DE POSTER EN EL CONGRESO QUE SERÁ REALIZADO DEL 23 AL 27 DE JULIO DE 2018 EN AMSTERDAM, PAÍSES BAJOS.Background: Th17 and Treg cells play a key role in HIV infection and mucosal defenses. A reduction of Th17 cells in the female genital mucosa (FGM) of HIV+ women has been previously described. Aim: To analyze the effects of antiretroviral treatment (ART) in these T-cell subsets in FGM in different groups of individuals.Methods: Cervical mononuclear cells (CMCs) and exocervical swabs from FGM were obtained from the following groups: HIV- (n=21), HIV+ART+ (n=32) and HIV+ART- (n=12). Cytokines (CKs) secreted by CMCs after stimulation with PMA+Ionomycin and chemokines in exocervical swabs were quantified by Cytometric Bead Array.Results: CMCs production of Th17-related-CKs (IL17A, IL17F, IL21 and IL22) and Treg-related-CKs (IL10 and TGF-β1) were evaluated. HIV+ART- group showed diminished proportions of positive responses for Th17-related-CKs compared to HIV- (IL17A, IL17F and IL21:p