Semen exosomes inhibit the Zika virus infection on dendritic cells

ANA LUZ PALETTA; FACUNDO DI DIEGO GARCÍA; ANA CEBALLOS; LUCIANA CASTILLO; JORGE GEFFNER; AUGUSTO VARESE; FEDERICO REMES LENICOV

Mar del Plata

Congreso; SAIC SAI SAFIS 2018; 2018

Semen exosomes inhibit the Zika virus infection on dendritic cellsSurprisingly, it has been reported sexual transmission of Zika virus (ZIKV), changing our view of arbovirus-host interaction. Infectious ZIKV persist in semen after symptoms onset at higher viral loads. Seminal plasma (SP) contains trillions of exosomes, small extracellular vesicles that mediate intercellular signaling. Knowing that semen is not merely a carrier for sexually transmitted diseases; our aim was to evaluate the role of semen exosomes (SE) on ZIKV infection of dendritic cells (DCs). SP and monocytes were obtained from healthy donors. SE were purified using size exclusion chromatography. DCs were obtained from monocytes (GM-CSF/IL-4, 5 days). ZIKV binding and infection was evaluated by qPCR, UFP/ml or by flow cytometry. Phenotype was analyzed by flow cytometry and cytokines production by ELISA.We infected DCs (2X105) with ZIKV (5x105ufp) in the presence or absence of SP(1/000-dil), SP-SE-depleted (1/1000-dil) or SE (200ug/ml) for 48hs. We observed the presence of SP abrogated the infection, while SP-SE-depleted not (p