INCREASED LPS-INDUCED PRODUCTION OF TNF BY MONOCYTES AFTER LONG-TERM EXPOSURE TO PROSTAGLANDIN E2 IS MEDIATED BY HOMOLOGOUS RECEPTOR DESENSITIZATION

FACUNDO DI DIEGO GARCÍA; JORGE GEFFNER; ANA LUZ PALETTA; ANA CEBALLOS; AUGUSTO VARESE; FEDERICO REMES LENICOV

Mar del Plata

Congreso; SAIC SAI SAFIS 2018; 2018

Sociedad Argentina de Microbiología

Prostaglandin E2 (PGE2) is a pleiotropic agent produced during acute and chronic inflammation. Presence of PGE2 during LPS stimulation of monocytes results on the inhibition of LPS-induced production of TNF. Previously, we have shown that long-term exposure of monocytes to PGE2 results in an increased ability to produce LPS-induced TNF. Here we analyzed the mechanism of this paradoxical effect. Monocytes were purified from peripheral blood using CD14+ magnetic microbeads. Monocytes were cultured for 15 hs in the presence of PGE2 (10-8M) or not (control) and then stimulated with LPS (25ng/ml). Production of TNF was evaluated by ELISA or intracytoplasmatic staining and flow cytometry.Monocytes pre-treated for 15 hs with PGE2 showed an increased in the production of TNF compared to control culture: 75+/-8 vs 56+/-7% TNF+ cells (n=9, p