LIMITED IL-2 BIOAVAILABILITY DURING PEDIATRIC RSV INFECTION

ERRA DIAZ , FERNANDO; HOLGADO, MARÍA PÍA; RAIDEN, SILVINA; GONZÁLEZ, FERNANDA; ARRUVITO, LOURDES; SANANEZ, INÉS; DE LILLO, LEONARDO; GEFFNER , JORGE

CABA

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS 2017; 2017

SAI-SAIC

It is well known that Respiratory Syncytial Virus, despite havingslight antigenic variation, repeatedly infects throughout life reflectinga defect in the development of immunological memory. In addition,we have reported that severe RSV infection of infants induced adramatic reduction in the frequency of peripheral blood regulatoryT cells (Tregs). Because IL-2 is crucial for both, the development ofT cell memory and the maintenance of Tregs, we analyzed whetherthe production and/or the availability of IL-2 was affected in RSV-infectedchildren (RSV-ch).Here, we aimed 1) to analyze if IL-2 addition increased the expressionof FOXP3 in CD4+ T cells from RSV-ch; 2) to determine the abilityof CD4+ T cells from RSV-ch to produce IL-2 upon in vitro stimulation;3) to analyze the serum levels of soluble IL-2Rα (sCD25); and4) to explore the response of CD4+ T cells from RSV-ch to sCD25.We observed that incubation of CD4+ T cells from RSV-ch withIL-2 (20 ng/ml) markedly increased the frequency of CD4+FOXP3+T cells (p