EXPRESSION OF PPARΓ AND PPARΑ ON A MACROPHAGIC CELL LINE STIMULATED WITH MYCOBACTERIUM TUBERCULOSIS

BETTINA BONGIOVANNI; ROCÍO DV FERNÁNDEZ; FEDERICO PENAS; ARIANA DÍAZ; OSCAR BOTTASSO; BAY MARIA LUISA; LUCIANO DATTILIO; GOREN NORA.; CEVEY AGATA

Mar del Plata

Congreso; Reunión Anual SAIC SAI SAFIS; 2018

SAIC SAI SAFIS

Tuberculosis (TB) is a major health problem characterized by animmune-endocrine imbalance: high plasma levels of cortisol andpro- and anti-inflammatory mediators, as well as lowered Dehydroepiandrosteronelevels. The etiologic agent, Mycobacterium tuberculosis (Mtb) is captured by lung macrophages (Mf), whoseactivation is required to cope with Mtb control, along with someform of bystander tissue damage. Glucocorticoids (GCs) are criticalelements to counterbalance the immune-inflammatory reaction,with peroxisome proliferator-activated receptors (PPAR) being alsoimplicated in this regard. The main forms of these receptors are:PPARα, PPARβδ and PPARγ. TB patients showed an increased expressionof PPARγ and PPARα transcript in their peripheral bloodmononuclear cells, the former being positively associated with circulatingcortisol and disease severity. Given this background, wenow investigated the expression of PPARγ and PPARα transcriptsby real time PCR in Mf (adherent human THP-1 cells, n=8) stimulatedwith Mtb (strain H37Rv killed by γ radiation -Mtbi-). There wasan increased in transcript expression of both receptors after 24 hrstimulation (p