CD32 expression promotes T cell activation in severely RSV-infected children

SANANEZ, INÉS; ERRA DIAZ, FERNANDO; HOLGADO, MARÍA PÍA; RAIDEN, SILVINA; URTASUN, MARCELA; GONZALEZ, FERNANDA; DE LILLO, LEONARDO; GEFFNER, JORGE; CAIROLI, HÉCTOR; DAVENPORT, CAROLINA; ARRUVITO, LOURDES

Mar del Plata

Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS); 2018

Background: Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis in young children worldwide. CD32 is a low-affinity Fc receptor with specificity for IgG antibodies commonly expressed on most myeloid cells. Preliminary studies of our group have shown an activating role for CD32 in CD4+ T cells from healthy adults. Here we aim to investigate the expression and function of CD32 in CD4+ T cells from RSV-severely infected children. Specific aims: 1- To analyze the expression of CD32 on CD4+ T cells from RSV-infected. 2- To examine whether CD4+ T cells bind aggregated IgG. 3 - To determine whether the expression of CD32 is associated with phenotypic markers. 4- To explore the biologic response of CD4+ T cells upon ligation with anti-CD32 antibody. Results: We observed an increased frequency of CD32+CD4+T cells in RSV-infected (16%±1.2; n=44) compared with healthy infants (5%±0.5; n=8), p