CYTOTOXIC CD8+CD127- T CELLS LEVES IN HIV INFECTED CHILDREN

BALBARYSKI, JEANETTE; ROFFE GEORGINA; GADDI, EDUARDO; SANCHEZ, MARÍA FLORENCIA; URIOSTE, ALEJANDRA; BARBONI, GRACIELA; CAPECCE, ESTEFANIA

Buenos Aires

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2017

Sociedad Argentina de Inmunología

Introduction: homeostatic expansion of naive and memory T cellssubsets require normal IL-7 alpha chain (CD127) expression levels.This molecule is exclusively expressed on T cells, and in HIV(+) adults patients, its down-regulation is associated with increaseCD8+ T cells apoptosis rate.Objective: to determine CD8+CD127- T cells levels in HIV infectedchildrenMaterial and methods: percentage of CD8+CD127- T cells levelsin 66 patients with HIV infection by vertical transmission, in antiretroviraltherapy, was studied by flow cytometry (FACSCalibur, BD)and specific monoclonal antibodies. Plasma HIV RNA viral load (VL)was determined by PCR NASBA. A control group (Co) of 10 healthychildren was also studied.Results: according to immunologic categories of ClassificationSystem for HIV infected children: LTCD4+percentage levels ≥ 25%(no evidence of suppression), < 24 % (evidence of moderate or severesuppression), and VL: detectable or not (> or < 50 IU/ml), childrenwere divided in four groups. Percentage levels of CD8+CD127-T cells in different groups were: A: (n: 8) (CD4