Limited IL-2 bioavailability during pediatric RSV infection

ARRUVITO, LOURDES; GONZALEZ, FERNANDA; SANANEZ, INÉS; GEFFNER, JORGE; DE LILLO, LEONARDO; RAIDEN, SILVINA; HOLGADO, MARÍA PÍA; ERRA DIAZ, FERNANDO

Conferencia; RSVVW CONFERENCE 2017 - 4TH ReSViNET MEETING; 2017

Background: Respiratory Syncytial Virus, despite having slight antigenic variation, repeatedly infects throughout life reflecting a defect in immunological memory. We have reported that severe RSV infection of infants induced a dramatic reduction in the frequency of regulatory T cells (Tregs). Because IL-2 is crucial for both, T cell memory and Tregs maintenance, we analyzed whether the availability of IL-2 was affected in RSV children (RSV-ch).Aims: 1) to analyze if IL-2 addition increased FOXP3 in CD4+T cells from RSV-ch; 2) to determine the ability of CD4+T cells from RSV-ch to produce IL-2; 3) to analyze the soluble IL-2Rα (sCD25) serum levels; 4) to explore the response of CD4+T cells to sCD25.Results: Incubation of CD4+T cells from RSV-ch with IL-2 increased the frequency of CD4+FOXP3+T cells (p