Activated macrophages and dendritic cells express clusterin

MERLOTTI IPPÓLITO, ANTONELLA; SABATTE, J; LOPEZ MALIZZIA, ALVARO; GEFFNER, J; CARREGAL, SOL

Buenos Aires

Congreso; Reunión Conjunta De Sociedades De Biociencias Lxv Reunión Anual De La Sociedad Argentina De Inmunología (SAI); 2017

SAI

Clusterin is a ubiquitous glycoprotein present in almost all tissuesand body fluids. Although clusterin appears to be involved in a varietyof physiological and pathological processes such as cell deathregulation, complement inhibition and Alzheimer?s disease, its functionis still controversial. Whereas cytosolic clusterin inhibits apoptosispromoting cell survival, the role of secretory clusterin (sCLU) remainsobscure. In this study, we analyzed the expression of clusterinin resting and activated human macrophages (M) and dendritic cells(DCs). To this aim, monocytes were differentiated into M or DCs byculturing monocytes during 7 days with M-CSF or GM-CSF plus IL-4, respectively. M or DCs (1x105/ml) were cultured in the absence(controls) or presence of LPS (10 ng/ml) for 48 h, and the concentrationof clusterin was determined in cell supernatants by ELISA.Resting M and DCs did not produce clusterin, while LPS-activatedM and DCs produced significant amounts of clusterin: 17 ± 3 and15 ± 3 ng/ml (media ± SE, n=5, p