SEMINAL VESICLE FLUID INCREASES THE EFFICACY OF INTRAVAGINAL HSV-2 VACCINATION

A. VARESE; M. GONZALEZ PRINZ; A. CEBALLOS; F. REMES LENICOV; J. GEFFNER; J. ODDI; A. MERLOTTI

Mar del Plata

Congreso; CONGRESO SAI; 2016

Semen induces biological actions on the female reproductive tissues directed to modulate the immune responseagainst paternal antigens. However, the influence of seminal plasma on the immune response against sexuallytransmitted pathogens has not been yet evaluated. Our aim was analyze whether the seminal vesicle fluid(SVF), might compromise the induction of a protective memory response induced by HSV-2 vaccination. SVFwas extracted post-mortem from 10-week-old BALB/c males. Female BALB/c mice were vaccinated IVAG withinactivated HSV-2 (1x104 pfu/15 ìl), without or with SVF (protein concentration 5 mg/ml). Thirty days later, micewere challenged IVAG wiht HSV-2 lethal dose. Mice were examined for clinical score and survival. Draining lymphnodes (DLN) and genital mucosa cells were recovered and phenotype and cytokines production was assessedby flow cytometry, ELISA, and qPCR. We observed that SVF-vaccinated mice showed 90% survival (control10%; n=20; p