PDL1 SIGNALING INHIBITS MACROPHAGE?S SUSCEPTIBILITY TO M. TUBERCULOSIS SPECIFIC CD8+ T CELL INDUCED APOPTOSIS

VECCHIONE, MARÍA BELÉN; SUAREZ, GUADALUPE; QUIROGA MARIA FLORENCIA

Mar del Plata

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA, LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016

Sociedad Argentina de Inmunologia

CD8+ T cells contribute to the optimal control of Tuberculosis infection by inducing apoptosis on infected macrophages. M. tuberculosis (Mtb) infection is associated to alternative activation of macrophages. We aimed to study the effect of macrophage activation on Mtb-specific CD8+ T cell induced apoptosis.Monocyte Derived Macrophages (MDMs) from healthy donors were activated by IFNγ or IL4 addition during 48 h and loaded with heat-killed Mtb or CEF peptide pool. Macrophages? phenotype was determined by HLA-DR, CD86, DC-SIGN and CD14 staining and flow cytometry. Then, MDMs were co-cultured with antigen-stimulated, autologous CD8+ T cells. Specific killing was assessed by 7-AAD staining. Data was analyzed by ANOVA followed by Bonferroni posttest.IFNγ activation of MDMs tended to decrease CD8-induced Mtb-specific apoptosis but it remained significant at a ratio of 1:1 (p