Expanded CD4+ CD25− FoxP3+ Regulatory T cell population in HIV-TB patients.

QUIROGA MARIA FLORENCIA; SUAREZ, GUADALUPE; LAUFER N; ANGERAMI, MATIAS TOMAS; SUED, OMAR; VECCHIONE, MARÍA BELÉN; BEN, GRACIELA

Seattle

Congreso; IMMUNOLOGY 2016; 2016

American Association of Immunologists

Tuberculosis (TB) and HIV alter the immune system, and coinfected (HIV-TB) individuals usually show deregulations in lymphocyte´s immune functions. We previously observed an increased frequency of ?unconventional? Treg (uTreg, CD4+CD25-FoxP3+) during HIV-TB. Therefore, we aimed to explore the phenotype and function of uTreg and conventional Treg subsets (cTreg, CD4+CD25+FoxP3+) among HIV-TB (n=34) patients and healthy donors (HD, n=30). We evaluated the expression of CD39, PD1, GITR and maturation status by flow cytometry in cTreg and uTreg. Also, IL-10, TGF-β, IFN-γ production and suppressive capacity were analyzed in cocultures of Tregs and effector lymphocytes. We found diminished expression of CD39 and higher levels of PD-1 on uTreg compared to cTreg in both HIV-TB (p