Identification of haplotypes in individuals with pathologies associated to Human T-Cell Lymphotropic Virus type 1 (HTLV-1) infection in Argentina.

BIGLIONE MM; CÁNEPA CB; FRAILE GONZÁLEZ SA; BERINI CA

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2016

Introduction: HTLV-1 is the causative agent of Adult T cellLeukemia/Lymphoma (ATLL) and HTLV-1 associated myelopathy(HAM). We propose to identify the HLA-A alleles related tosusceptibility/protection for ATLL and HAM in cases from Argentina.Materials and methods: A total of 29 cases, 7 with ATLL and22 with HAM were studied. DNA was extracted, proviral load (PV)determined by qPCR and HLA-A by PCR and sequencing.Results: The PV was significantly lower in asymptomaticscompared to pathologies. Eight polymorphisms of HLA-A wereobserved: A*02, A*33, A*31, A*29, A*11, A*68, A*23 and A*24.HLA-A*23, A*24 and A*68 were only observed in ATLL, while A*11and A*29 in HAM. HLA-A*02, A*31 and A*33 were present in bothpathologies (p= 0.38; p=0.84 and p=0.36). In HAM, HLA-A*02 wasmore frequent, followed by A*33, with no significant difference(p=0.09). When comparing polymorphisms with 19 asymptomatics,A*02 was more frequent in the former (p=0.0092) while A*33in individuals with pathologies (p=0.045). Individuals bearing theA*31 allele had a significantly higher CPV compared to the onesbearing A*02 (p=0.0488).Discussion: In Argentina, A*02, A*24 and A*26 were observedin seronegative individuals with frequencies of 48.2%, 8.9% and5.3%, respectively. No significant differences for A*02 and A*24were observed when compared to our results in individuals withpathologies and asymptomatics; although HLA-A*02 was morefrequent in the former, and A*26 was absent. In Jamaica, Japanand Brazil HLA-A*02 was described as protective both for ATLL andHAM, in concordance to our results. HLA-A*33 has been previouslydescribed as protector for ATLL in Jamaica, while in our populationit was associated to individuals with pathologies, especially withHAM. More studies are necessary to confirm these results and beused as a prognosis guide for the development of the pathologies.