Phenotypical and functional evaluation of regulatory T cells expanded CD4+CD25-FoxP3+ population in HIV-TB patients

MATÍAS TOMAS ANGERAMI; MARÍA BELÉN VECCHIONE; GUADALUPE SUAREZ; OMAR SUED; GRACIELA BEN; NATALIA LAUFER; MARIA FLORENCIA QUIROGA

Ciudad Autónoma de Buenos Aires

Congreso; IV LASID Meeting, LXIII Argentinean Immunology Society Meeting and II French-Argentinean Immunology Meeting.; 2015

IV LASID, LXIII Argentinean Immunology Society and II French-Argentinean Immunology

Tuberculosis (TB) and HIV infection lead to adiscouraging panorama worldwide. Both pathogens synergisticallyaltered the immune system, and coinfected (HIV-TB) individualsusually demonstrated deregulation in lymphocyte populations andimmunological markers. We previously showed an increasedfrequency of ?unconventional? Treg (uTreg, CD4+CD25-FoxP3+)during coinfection. We aimed to explore differences betweenuTreg and conventional Treg subsets (cTreg,CD4+CD25+FoxP3+) among HIV-TB (n=34) patients and healthydonors (HD, n=30). We evaluated surface expression of CD39,PD1, GITR and maturation status by CD27 and CD45RA stainingon cTreg and uTreg. Also, cytokine production (IL-10, TGF-β,IFN-g) and suppressive capacity were analyzed. We founddiminished expression of CD39 and higher levels of PD-1 onuTreg compared with cTreg in both HIV-TB (p