Classical or alternative activation decreases macrophage susceptibility to CD8+ T cell killing

SUAREZ, GUADALUPE; VECCHIONE, MARÍA BELÉN; ANGERAMI, MATIAS TOMAS; QUIROGA MARIA FLORENCIA

Congreso; IV LASID Meeting LXIII, Argentinean Immunology Society Meeting and II French-Argentinean Immunology Meeting; 2015

SAI-LASID

CD8+ T cells contribute to the optimal control of Tuberculosis infection by inducing apoptosis on infected macrophages. Mtb infection is associated to alternative activation of macrophages. We aimed to study the effect of macrophage activation on Mtb-specific CD8+ T cell induced apoptosis.Monocyte Derived Macrophages (MDMs) from healthy blood donors were classically activated by IFN-γ or alternatively activated by IL-4 addition during 48 h and loaded with M. tuberculosis (Mtb) antigens. Macrophages phenotype was determined by HLA-DR, CD86, DC-SIGN and CD14 staining and flow cytometry analysis. Then, MDMs were co-cultured with previously Mtb-stimulated, autologous CD8+ cells at different CD8:MDM ratios. Specific killing was assessed after 3h by Annexin V and 7-AAD staining and IFN-γ production by CD8+ cells was assessed after 18h by ELISPOT assay.CD8-induced apoptosis of unactivated MDMs was Mtb-specific (p