PCR fingerprinting of rRNA intergenic spacer regions (ISRs) for molecular characterization of environmental bacteria isolates

MARTÍNEZ MA; SINERIZ F

Environmental Microbiology Protocols

Humana Press

Año: 2004; p. 159 - 166

As a result of screening programs to isolate strains of biotechnological interest or in diversity studies of cultivable bacteria, it is necessary a fast and easy procedure to characterize and differentiate them, especially when a large number of isolates should be considered. Among PCR fingerprinting methods, ISR-PCR have been successfully used in our laboratory as a fast and reproducible procedure to cluster isolated bacteria according to different band patterns.