Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans

MARTINEZ M. ALEJANDRA; DELGADO OSVALDO D.; BRECCIA JAVIER D.; BAIGORI MARIO D.; SINERIZ FAUSTINO

EXTREMOPHILES

SPRINGER TOKYO

Lugar: Tokyo; Año: 2002 p. 391 - 395

1431-0651

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US; mso-fareast-language:EN-US;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Bacillus sp. MIR32 has been isolated using xylan as the only carbon source and its xylanolytic enzyme has been extensively studied. Biochemical analysis first related this strain to amyloliquefaciens species, but further studies based on comparison of 16S rDNA sequences, G+C content and DNA-DNA hybridization revealed that MIR32 strain is highly related to halodurans species. PCR fingerprinting methods directed towards spacers into rDNA and tDNA genes were also assayed and compared with that of B. halodurans DSM 497T, which allowed us to obtain a more complete molecular characterization of the native strain. Although among alkaliphilc bacilli competence development has not been experimentally demonstrated, in this work both B. halodurans MIR32 and DSM 497T strains were transformed according to a simple procedure developed in our laboratory obtaining 102 to 103 stable transformants per mg of plasmid DNA.