Lipid extracts of Nicotiana glauca Graham (Solanaceae) induce apoptosis in human rhabdomyosarcoma cells.

MUSSO, FLORENCIA; PRONSATO, LUCÍA; MILANESI, LORENA; VASCONSUELO, ANDREA; FARAONI, M.BELÉN

RODRIGUESIA

MMM

Lugar: Rio de Janeiro; Año: 2020

0370-6583

AbstractRhabdomyosarcoma (RMS) is the most common soft-tissue tumor in children andadolescents. It is originated in normal skeletal muscle, from myogenic cells that have failedto fully differentiate, and usually has a poor prognosis. Current RMS therapy has manyadverse effects. Hence, new treatments are needed. Various pharmacological propertieshave been demonstrated in species of the Solanaceae family, such as analgesics,antineoplastic, antimicrobial and antiparasitic. We performed an ethanolic extraction fromleaves of Nicotiana glauca (Solanaceae) and the extract was successively partitioned withn-hexane, chloroform and ethyl acetate. We evaluated the effects of extracts onrhabdomyosarcoma cells, we found that the extracts trigger apoptosis. By bio-guidedfractionation assays, we identified the apoptotic agents. Morphological assessment afterapoptotic cell induction of cultured cells, mitochondrial and nuclear morphology byMitotracker and 4,6-diamidino-2-phenylindole (DAPI) staining respectively, wereanalyzed in fluorescent microscopy. The capacity of the cells to migrate or proliferate wasanalyzed by the Petit assay followed by methylene blue stained. NMR and GC-MSspectrometry were used to identify palmitic acid and scopoletin as one of thephytochemicals responsible for the observed effects. These results indicate that thesecompounds are apoptotic inducers and they could be useful as chemotherapeutic agentsagainst muscle tumors.Key words: Apoptosis, Nicotiana glauca, Palmitic acid, Rhabdomyosarcoma, Scopoletin.