MITOCHONDRIAL FUNTIONALITY AND ROS PRODUCTION ARE COMPROMISED IN Arabidopsis thaliana invh MUTANTS

BATTAGLIA M; MARTIN MV; SALERNO G; MARTÍNEZ NOËL G

Ciudad Autónoma de Buenos Aires

Congreso; SAIB 2017; 2017

Sociedades de Biociencias - SAIB 2017

Sucrose (Suc) plays a central role in plant growth and development, and inresponse to environmental stresses. Alkaline/Neutral-Invertases (A/N-Invs)(localized in the cytosol, mitochondria and/or plastids) are sucrose hydrolyzingenzymes with optimal pH between 6.8 and 8.0. Currently, there are manyquestions about the role of the organellar isoforms. Previous studies in A.thaliana suggested that two mitochondrial A/N-Invs (isoforms A and C) havesignificant roles in root growth and development, and in abiotic stressadaptation. However, A/N-InvH is a third isoform whose role is still unknown. Inthe present work, we showed using fluorescence and confocal microscopy thatinvh (a mutant lacking A/N-InvH) exhibits lower mitochondrial membranepotential than wild-type plants. Moreover, ROS production in invh roots isimpaired not only in salt and ABA treated plants but also in control conditionswhen are compared with wild-type plants. Taking together our data, weconclude that A/N-InvH plays a relevant role in mitochondrial functional statusand in ROS production either under control or stress conditions. Supported byCONICET, ANPCyT (PICT 1288), UNMdP (EXA645) and FIBA.Keywords: SUCROSE, ALKALINE/NEUTRAL-INVERTASES, ROS,MITOCHONDRIA