Comparative analysis of alkaline/neutral invertases among plants

L. LECHNER; M. BATTAGLIA; M.V. MARTIN; G. MARTINEZ NOËL; G.L. SALERNO

Mar del Plata

Congreso; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB 2015); 2015

Sociedad Argentina de Investigación Bioquímica

Sucrose (Suc) plays a central role in plant growthand development, and in response to environmental stresses.Alkaline/Neutral-Invertases (A/N-Inv) (localized in the cytosol, mitochondriaand/or in plastids) hydrolyze Suc to hexoses for maintenance functions. Recentlysignificant roles in root morphogenesis, germination, photosynthetic apparatusbiosynthesis, abiotic and biotic stress tolerance have been revealed. A/N-Inv multigenefamilies have been reported in A. thaliana (9 genes), O. sativa (8),P. trichocharpa (16), V. vinifera (9), L. japonicus (7) and M. esculenta (11). In the presentstudy we identified the A/N-Inv family in the wheat genome, analyzed itsstructure, phylogeny and expression patterns, which were compared with otherplants. Similarly to rice, we identified 4 and 3 putative genes coding forcytosolic (Ta-NIN5 to 8) and organelle isoforms (Ta-NIN1 to 4), respectively,whose sequences were categorized into the two major clades described. Expressionanalyses showed that genes coding for cytosolic isozymes expressed in roots andleaves, and were differentially regulated in response to cold and droughtstress. We conclude that organellar wheat A/N-Invs are conserved and common todistantly related plants, and some of the cytosolic isoforms are induced inresponse to environmental stresses. Supportedby CONICET, ANPCyT (PICT 1288), UNMdP and FIBA.