Does soybean cultivation generate losses of AMF diversity?

FAGGIOLI, VS; COVACEVICH, F; HERNANDEZ GUIJARRO, K; CABELLO, MN

Zurich

Simposio; 33 New Phytologist Symposium; 2014

New Phytologist

We assessed the effect of soybean crop intensity on native arbuscular mycorrhizal fungi (AMF)diversity. Soybean rhizospheric soil (of different-contrasting land use intensity) was collected from 120 sites according to a gridline design in an agricultural area of 12,000 km2 from Argentine pampas and taxonomical spore identification was made. Furthermore, we select the most contrasting conditions: High (intensive soybean crop); Med (soybean crop rotated with forage), Low (soybean crop after clearing native shrubs) and molecular analysis of AMF spores was conducted. Specific fragments of 25S rDNA were PCR amplified (LSU 0061/0599 and RK4f/RK7r, primers specific for Glomus) and single stranded conformation polymorphisms (SSCP) was performed. We identified 32 AMF species and 14 genera. Acaulospora and Glomus were predominant in High (A. scrobiculata and G. fuegianum, respectively). In Med and Low conditions, predominant AMF genera were: Glomus, Septoglomus, Acaulospora, Funneliformis, Ambispora and Diversispora. The SSCP analysis revealed high AMF diversity belonging to Glomus genera at High condition followed by Med one. Some bands appeared at SSCP gel for Low condition but they were not present in High or Med. We conclude that soybean cultivation generated losses of AMF diversity and contributed to predominance of Glomus species depending on intensity of land use.2 from Argentine pampas and taxonomical spore identification was made. Furthermore, we select the most contrasting conditions: High (intensive soybean crop); Med (soybean crop rotated with forage), Low (soybean crop after clearing native shrubs) and molecular analysis of AMF spores was conducted. Specific fragments of 25S rDNA were PCR amplified (LSU 0061/0599 and RK4f/RK7r, primers specific for Glomus) and single stranded conformation polymorphisms (SSCP) was performed. We identified 32 AMF species and 14 genera. Acaulospora and Glomus were predominant in High (A. scrobiculata and G. fuegianum, respectively). In Med and Low conditions, predominant AMF genera were: Glomus, Septoglomus, Acaulospora, Funneliformis, Ambispora and Diversispora. The SSCP analysis revealed high AMF diversity belonging to Glomus genera at High condition followed by Med one. Some bands appeared at SSCP gel for Low condition but they were not present in High or Med. We conclude that soybean cultivation generated losses of AMF diversity and contributed to predominance of Glomus species depending on intensity of land use.