INVESTIGADORES
ZANETTI Maria Eugenia
congresos y reuniones científicas
Título:
Dissecting the molecular response of common bean to Rhizobium etli with next-generation sequencing technologies (NGST)
Autor/es:
VIRGINIA DALLA VIA, CANDELA NARDUZZI, MARCOS LANCIA, MARIO AGUILAR, MARÍA EUGENIA ZANETTI Y FLAVIO BLANCO
Lugar:
Chascomús
Reunión:
Congreso; XV Jornadas Anuales de la Sociedad Argentina de Biología; 2013
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
Legume plants establish a mutualistic symbiosis with soil
bacteria that convert N2 to forms that can be directly incorporated into
the metabolism. Understanding the molecular basis of this process is key to
alleviate the use of chemical fertilizers, preventing the environmental
pollution associated to modern agricultural practices. In this work, we used
next generation sequencing technologies to unravel two aspects of the symbiotic
interaction, the root transcriptional response modulated by bacterial signals and
the molecular basis of the selection of the most efficient strains by the
plant. To achieve the first objective, mRNA from root tissue of common bean
plants inoculated with the wild type
strain of R. etli or with mutant
strains that are impaired in the synthesis of the Nod Factor, the exopolysaccharide
and, the lipopolysaccharide I were used to
construct Illumina libraries that have been sequenced. For the second
objective, we took advantage of the evolution of common bean in two centers of
genetic diversification, the Mesoamerican and the Andean regions, which
resulted in the capability of Mesoamerican accessions to preferentially associate
with R. etli strains that are prevalent in Mesoamerican soils. This specific
association is correlated with a more efficient nodulation and nitrogen
fixation. Illumina libraries were prepared to compare the transcriptome of
roots inoculated with a more efficient strain with that of roots inoculated
with the less efficient one. Expression of selected genes was confirmed by
RT-qPCR. Our current progress in NGST analysis, as well as further implication
of our findings, will be discussed.