INVESTIGADORES
ZANETTI Maria Eugenia
congresos y reuniones científicas
Título:
NFYC1, a transcription factor required for nodule organogenesis, interacts with a receptor like kinase and a GRAS transcriptional regulator
Autor/es:
JOAQUÍN CLÚA, CAROLINA RÍPODAS, MARINA BATAGLIA, MARÍA EUGENIA ZANETTI AND FLAVIO BLANCO
Lugar:
Chascomús
Reunión:
Congreso; XV Jornadas Anuales de la Sociedad Argentina de Biología; 2013
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
Common bean (Phaseolus
vulgaris) establishes a nitrogen fixing association with its partner Rhizobium
etli. In this interaction, host-dependant competitiveness has been observed,
in which accessions from the Mesoamerican region are more efficiently and
preferentially nodulated by strains that are predominant in the same
geographical region. A C subunit of the heterotrimeric nuclear factor Y
(NF-Y), PvNF-YC1, was identified as a gene required for nodule
organogenesis and bacterial infection that contributes to this preferential
association. In order to identify proteins that can physically interact with this
transcription factor, a yeast two hybrid screening was performed using PvNF-YC1
as a bait and a cDNA library from root tissue inoculated with R. etli. A
total of eight clones that potentially interacts with NF-YC1 were isolated and
sequenced. Among them, one encodes a receptor-like
kinase and another clone encodes a transcriptional regulator of the GRAS family. Interaction of PvNF-YC1 with these gene
products has been confirmed by retransformation of yeast, and it is being
evaluated in planta by bimolecular
fluorescent complementation assays in Agrobacterium-infiltrated Nicotiana
benthamiana leaves and co-immunoprecipitation assays. Function of these
PvNF-YC1 interacting proteins in nodulation efficiency and bacterial infection
is being evaluated by both RNAi and overexpression. This will help to elucidate
the signal transduction pathway specifically activated in Mesoamerican common
bean in response to its cognate R. etli strain.