INVESTIGADORES
ZANETTI Maria Eugenia
congresos y reuniones científicas
Título:
Regulatory small RNAs involved in the symbiosis between Phaseolus vulgaris and Rhizobium etli
Autor/es:
MÉLISSE CASTAINGTS, CAROLINA RÍPODAS, O. MARIO AGUILAR, FLAVIO BLANCO AND MARÍA EUGENIA ZANETTI
Lugar:
Chascomús
Reunión:
Congreso; XV Jornadas Anuales de la Sociedad Argentina de Biología; 2013
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
Common
bean (Phaseolus vulgaris) establishes a symbiotic interaction with Rhizobium etli, resulting in the
formation of root nitrogen-fixing nodules. Interestingly, beans from the
Mesoamerican center of genetic diversification are more efficiently and
preferentially nodulated by strains that are predominant in the same
geographical region. Our group has identified several transcription factors
that are determinant for this preferential interaction. One of them encodes an
A subunit of the heterotrimeric transcription factor NF-Y named PvNF-YA1. PvNF-YA1 is strongly expressed at early
stages of the interaction with the highly efficient strain of R. etli, but not with the less efficient
one, suggesting a special function in strain specificity. In silico analysis revealed that PvNF-YA1 could be regulated by two microRNAs (miR169b and miR169d) through
their putative target sites, which are present in the 3?UTR of the PvNF-YA1 transcript. Overexpression of a
miR169 resistant version of PvNF-YA1
resulted in an increase in the number of nodules formed by the less efficient
strain. RT-PCR analysis revealed that miR169d precursor is specifically and
highly expressed in young nodules formed by the highly efficient strain, where
two different spliced variant where identified. Simultaneously, in order to get
access to global changes in small RNAs (sRNAs) abundance during this symbiotic
interaction, we have constructed sRNAs libraries of roots inoculated with the more
or the less efficient strain that will be subjected to Illumina sequencing.
This approach will allow us to identify new sRNAs involved in the
preferentially association.