A monomeric G-protein of the Rab family is required for root hair formation and pre-infection stages in the common bean-rhizobium symbiotic association

BLANCO FA, PELTZER MESCHINI E, ZANETTI ME, AGUILAR OM.

PLANT CELL

AMER SOC PLANT BIOLOGISTS

Año: 2009 vol. 21 p. 2797 - 2810

1040-4651

Legume plants are able to establish a symbiotic relationship with soil bacterial from the genus Rhizobium, leading to the formation of nitrogen fixing root nodules. Successful nodulation requires both the formation of infection threads (ITs) in the root epidermis and the activation of cell division in the cortex to form the nodule primordium. This study describes the characterization of PvRabA2a, a common bean cDNA previously isolated as differentially expressed in root hairs infected with R. etli, which encodes a protein highly similar to small GTPases of the RabA2 subfamily. This gene is expressed in roots, particularly in root hairs, where the protein was found to be associated to vesicles that move along the cell. The role of this gene during nodulation has been studied in Phaseolus vulgaris composite plants using a reverse genetic approach. Examination of root morphology in PvRabA2a RNAi plants revealed that the number and length of the root hairs were severely reduced in these plants. Upon inoculation with R. etli, nodulation was completely impaired and no induction of early nodulation genes (ENODs) was detected in silenced hairy roots. Moreover, PvRabA2a RNAi plants failed to induce root hair deformation and to initiate ITs, indicating that morphological changes that precede bacterial infection are compromised in these plants. Early molecular responses, as ERN1, ENOD40 and Hap5 induction are abolished in PvRabA2a RNAi roots. We propose that PvRabA2a acts in polar growth of root hairs and is required for reorientation of root hair growth axis during bacterial infection.