INVESTIGADORES
ZANETTI Maria Eugenia
artículos
Título:
Selective recruitment of mRNAs and miRNAs to
Autor/es:
MAURICIO ALBERTO REYNOSO, FLAVIO ANTONIO BLANCO, JULIA BAILEY-SERRES2,MARTÍN CRESPI, MARIA EUGENIA ZANETTI
Revista:
PLANT JOURNAL
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2013 vol. 77 p. 289 - 301
ISSN:
0960-7412
Resumen:
Translation of mRNAs is a key regulatory step that contributes to the coordination and modulation of
eukaryotic gene expression during development or adaptation to the environment. mRNA stability or translatability
can be regulated by the action of small regulatory RNAs (sRNAs), which control diverse biological
processes. Under low nitrogen conditions, leguminous plants associate with soil bacteria and develop a
new organ specialized in nitrogen fixation: the nodule. To gain insight into the translational regulation of
mRNAs during nodule formation, the association of mRNAs and sRNAs to polysomes was characterized in
roots of the model legume Medicago truncatula during the symbiotic interaction with Sinorhizobium meliloti.Medicago truncatula during the symbiotic interaction with Sinorhizobium meliloti.
Quantitative comparison of steady-state and polysomal mRNAs for 15 genes involved in nodulation
identified a group of transcripts with slight or no change in total cellular abundance that were significantly
upregulated at the level of association with polysomes in response to rhizobia. This group included mRNAs
encoding receptors like kinases required either for nodule organogenesis, bacterial infection or both, and
transcripts encoding GRAS and NF-Y transcription factors (TFs). Quantitative analysis of sRNAs in total and
polysomal RNA samples revealed that mature microRNAs (miRNAs) were associated with the translational
machinery, notably, miR169 and miR172, which target the NF-YA/HAP2 and AP2 TFs, respectively. Upon
inoculation, levels of miR169 pronouncedly decreased in polysomal complexes, concomitant with the
increased accumulation of the NF-YA/HAP2 protein. These results indicate that both mRNAs and miRNAs
are subject to differential recruitment to polysomes, and expose the importance of selective mRNA translation
during root nodule symbiosis.
NF-YA/HAP2 and AP2 TFs, respectively. Upon
inoculation, levels of miR169 pronouncedly decreased in polysomal complexes, concomitant with the
increased accumulation of the NF-YA/HAP2 protein. These results indicate that both mRNAs and miRNAs
are subject to differential recruitment to polysomes, and expose the importance of selective mRNA translation
during root nodule symbiosis.