Lung fixation in the state of inhalation for MRI with hyperpolarized gases

P. BLÜMLER; ANGELIKA THOMAS-SEMM; STEFAN REUSS; A. MORBACH; R. H. ACOSTA; J. SCHMIEDESKAMP; W. SCHREIBER

Toronto, Canadá

Congreso; 11th International Society of Magnetic Resonance in Medicine Meeting; 2003

MRI investigations of lungs require reproducible models for sequence evaluation. The procedures for the fixation of entire lungs of small rodents up to pigs arepresented. Therefore the lung/heart complex is excorporated and filled with various liquids for fixation using a lower pressure on the outside. Finally it is driedand can be stored in an non-collapsed state of inhalation. Light-, SEM-Microscopy and MRI techniques using hyperpolarised 3He were employed to verify theintact state of the alveolae on a microscopic level and the entire morphology of the entire lung.