An unprecedented metabolic study characterizes hypertension and demonstrates how intervention with broccoli microgreens can modify its evolution

DANIELA RAMIREZ; ROBERTO BAFUMO; SEBASTÁN GARCÍA MENÉNDEZ; FERNANDO DARÍO CUELLO CARRIÓN; NATALIA PRADO; EMILIANO DIEZ; WALTER MANUCHA

San Juan

Congreso; XLI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2023

Sociedad de Biología de Cuyo

The current socioeconomic and environmental context poses global health challenges due to the high incidence of chronic diseases like diabetes, obesity, and hypertension (HT). A promising strategy is to promote healthy diets based on sustainable production systems, where micro-vegetables or "microgreens" (MG) from the Brassicaceae family stand out for their bioactive value and content of phenolic compounds, glucosinolates, carotenoids, tocopherols, and ascorbic acid, with anti-cancer and cardioprotective properties. Indole-3-carbinol (I3C) and anthocyanins have been shown to reduce oxidative stress and cardiovascular risk. Although progress has been made in studying phytochemicals and their relationship with cardiovascular diseases, studies on plasma metabolites as a "biochemical phenotype" are crucial for more effective treatment. Metabolomics, an analytical, diagnostic, and therapeutic tool, provides a holistic view of biological data, analyzing changes after food intake and identifying chemical markers and affected metabolic pathways. The present study aims to evaluate a dietary intervention on an animal model of HT using a specific dose of MG from the Brassicaceae family (MGB). In this sense, we hypothesize that this diet will positively affect oxidative, inflammatory, and phenotypic markers characteristic of hypertensive disease. We used normotensive (WKY) and hypertensive (SHR) rats with a diet that contained 5% MGB for different periods (1 day = acute and 30 days = chronic), CICUAL 232/23. Hemodynamic parameters and oxidative-inflammatory markers were evaluated, and histological and metabolomic analyses were performed. The results revealed that the chronic MGB diet significantly reduced systolic blood pressure in SHR (p