Anti-inflammatory, antioxidant, antihypertensive, and antiarrhythmic effect of indole-3-carbinol, a phytochemical derived from cruciferous vegetables

NATALIA PRADO; DANIELA RAMIREZ; LUCIANA MAZZEI; MICAELA PARRA; MARIANA CASAROTTO; JUAN PABLO CALVO; DARÍO CUELLO-CARRIÓN; AMIRA PONCE ZUMINO; EMILIANO DIEZ; ALEJANDRA CAMARGO; WALTER MANUCHA

Heliyon

London : Elsevier Ltd, [2015]-

Lugar: Londres; Año: 2022 vol. 8 p. 1 - 8

2405-8440

Background: Cardiovascular inflammation and oxidative stress are determining factors in high blood pressure. Indole-3-carbinol (I3C) is a bioactive compound derived from cruciferous vegetables (broccoli and cabbage) with potential antiinflammatory and antioxidant effects. However, the full implications of this compound on the modulation of cardiovascular oxidative-inflammatory markers are still poorly described. Objectives: To establish the effects of I3C on the oxidative-inflammatory-proarrhythmic conditions associated with hypertension. Materials: Histological, biochemical, molecular, and functional aspects were evaluated in: 1) mouse BV-2 glial cells subjected to oxidative-inflammatory damage by lipopolysaccharides (LPS, 100 ng/ml) 50 μM in the presence or absence of 40 μM I3C (n=5); 2) male spontaneously hypertensive rats (SHR) and Wistar Kyoto rats that received I3C (2000 dpi/day, orally) during the first 8 weeks of life (n=10); and 3) isolated rat hearts submitted to 10 min regional ischemia and 10 min reperfusion. Results: 1) LPS induced oxidative stress and increased inflammatory markers; however, I3C reversed both conditions (IL-6, TNF-α, NADPH activity, NO, iNOS, Hsp70; p