INVESTIGADORES
SELDES Veronica
artículos
Título:
Application of the iPLEXTM Gold SNP genotyping method to the analysis of Amerindian ancient DNA samples: benefits for ancient population-based studies
Autor/es:
MENDISCO FANNY, KEYSER CHRISTINE, CLÉMENCE HOLLARD,VERONICA SELDES, NIELSEN AXEL, CRUBÉZY ERIC, LUDES BERTRAND
Revista:
ELECTROPHORESIS
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2011 vol. 32 p. 386 - 393
ISSN:
0173-0835
Resumen:
Important developments in the matrix-assisted laser desorption/ionization time-of-flight
mass spectrometry (MALDI-TOF MS) technique have generated new perspectives
regarding SNP genotyping, which are particularly promising for ancient populationbased
studies. The main aim of the present study was to investigate the application of a
MALDI-TOF MS-based SNP genotyping technique, called iPLEXs Gold, to analyze
Amerindian ancient DNA samples. The first objective was to test the sensitivity of the
method, which is recommended for DNA quantities between 10 and 5 ng, for ancient
biological samples containing DNA molecules that were degraded and present in minute
quantities. The second objective was to detail the advantages of this technique for studies
on ancient populations. Two multiplexes were designed, allowing the major Amerindian
mitochondrial and Y haplogroups to be determined simultaneously. This analysis has
never been described before. Results demonstrated the reliability and accuracy of the
method; data were obtained for both mitochondrial and nuclear DNA using picogram
(pg) quantities of nucleic acid. This technique has the advantages of both MS and
minisequencing techniques; thus, it should be included in the protocols for future
ancient DNA studies.
Amerindian ancient DNA samples. The first objective was to test the sensitivity of the
method, which is recommended for DNA quantities between 10 and 5 ng, for ancient
biological samples containing DNA molecules that were degraded and present in minute
quantities. The second objective was to detail the advantages of this technique for studies
on ancient populations. Two multiplexes were designed, allowing the major Amerindian
mitochondrial and Y haplogroups to be determined simultaneously. This analysis has
never been described before. Results demonstrated the reliability and accuracy of the
method; data were obtained for both mitochondrial and nuclear DNA using picogram
(pg) quantities of nucleic acid. This technique has the advantages of both MS and
minisequencing techniques; thus, it should be included in the protocols for future
ancient DNA studies.
s Gold, to analyze
Amerindian ancient DNA samples. The first objective was to test the sensitivity of the
method, which is recommended for DNA quantities between 10 and 5 ng, for ancient
biological samples containing DNA molecules that were degraded and present in minute
quantities. The second objective was to detail the advantages of this technique for studies
on ancient populations. Two multiplexes were designed, allowing the major Amerindian
mitochondrial and Y haplogroups to be determined simultaneously. This analysis has
never been described before. Results demonstrated the reliability and accuracy of the
method; data were obtained for both mitochondrial and nuclear DNA using picogram
(pg) quantities of nucleic acid. This technique has the advantages of both MS and
minisequencing techniques; thus, it should be included in the protocols for future
ancient DNA studies.