DNA DAMAGE INDUCED BY PACLITAXEL AND DNA REPAIR CAPABILITY OF PERIPHERAL BLOOD LYMPHOCYTES AS EVALUATED BY THE ALKALINE COMET ASSAY

BRANHAM MT ; NADIN SB; VARGAS ROIG LM; CIOCCA DR

MUTATION RESEARCH. GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

Elsevier

Lugar: Oxford; Año: 2004 vol. 560 p. 11 - 17

1383-5718

This study was designed to assess whether the chemotherapeutic drug paclitaxel can induce DNA damage in peripheral blood lymphocytes of human healthy donors, and to evaluate if such damage could be repaired. Venous blood was collected by routine venipuncture, the lymphocytes were isolated and cultured and then treated with 100nM, 500nM, 10microM, and 30microM of taxol for 4h. The alkaline comet assay technique was used to quantify the level of DNA damage and the DNA repair in lymphocytes. A significant increase in DNA damage was achieved when the cells were incubated with paclitaxel concentrations of 10microM or above. To test the DNA repair capability, the lymphocytes were allowed to recover for 2, 4, 6, and 24h. The DNA damage was almost completely repaired after 24h of incubation demonstrating a time-dependent repair capability. In conclusion, we demonstrate that paclitaxel induces DNA damage in peripheral blood lymphocytes and that this damage can be repaired.