Detection of an international multiresistant clone belonging to sequence type 654 involved in the dissemination of KPC-producing Pseudomonas aeruginosa in Argentina.

PASTERÁN FERNANDO; FACCONE, DIEGO; GÓMEZ, SONIA ALEJANDRA; DE BUNDER SABRINA; SPINELLI FEDERICO; RAPOPORT, MELINA; PETRONI, ALEJANDRO; GALAS, MARCELO; CORSO, ALEJANDRA

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2012 vol. 67 p. 1291 - 1293

0305-7453

KPC producers are mostly Enterobacteriaceae, but Pseudomonas aeruginosa have also been reported and mostly identified in the American continent. However, it is unknown if this is due to the spread of epidemic strains since the multi-locus sequence type has not been provided in most of those reports. The aim of this work was to characterize KPC-producing P. aeruginosa isolated in Argentina from 2006 to June 2011.A subset (n¼30) of suspected isolates was submitted to the NRL where blaKPC-2 was confirmed in all.XbaI PFGE analysis, using previously described criteria,5 revealed that all but one of the isolates belonged to a single pulsotype. Multilocus sequence typing of one strain of each PFGE type revealed that the dominant clone belonged to ST654, while the unique strain of PFGE type B, belonged to ST162. All isolates belonging to ST654 harboured blaKPC-2 in Tn4401b, which was confirmed by sequencing. The ST162 isolate harboured blaKPC-2 in Variant 1a. Unlike other Latin American regions, such as Puerto Rico and Colombia, where different PFGE types and STs have been identified,1,4 the dissemination of KPC-producing P. aeruginosa in Argentina is mainly associated with a single clone.