INVESTIGADORES
GOMEZ Sonia Alejandra
artículos
Título:
Differential expression of function-related antigens on blood monocytes in children with hemolytic uremic syndrome.
Autor/es:
GABRIELA C. FERNÁNDEZ; MARÝÍA V. RAMOS; SONIA A. GÓMEZ; GRACIELA I. DRAN; RAMÓN EXENI; MARTA ALDUNCÍN; IRENE GRIMOLDI; GRACIELA VALLEJO; CHRISTIAN ELÍAS-COSTA; MARTÍN A. ISTURIZ; MARINA S. PALERMO
Revista:
JOURNAL OF LEUKOCYTE BIOLOGY
Editorial:
FEDERATION AMER SOC EXP BIOL
Referencias:
Año: 2005 vol. 78 p. 853 - 861
ISSN:
0741-5400
Resumen:
Monocytes (Mo) mediate central functions
in inflammation and immunity. Different subpopulations
of Mo with distinct phenotype and
functional properties have been described. Here,
we investigate the phenotype and function of peripheral
Mo from children with hemolytic uremic
syndrome (HUS). For this purpose, blood samples
from patients in the acute period of HUS (HUS AP)
were obtained on admission before dialysis and/or
transfusion. The Mo phenotypic characterization
was performed on whole blood by flow cytometry,
and markers associated to biological functions
were selected: CD14 accounting for lipopolysaccharide
(LPS) responsiveness, CD11b for adhesion,
Fc receptor for immunoglobulin G type I
(FcRI)/CD64 for phagocytosis and cytotoxicity,
and human leukocyte antigen (HLA)-DR for antigen
presentation. Some of these functions were
also determined. Moreover, the percentage of
CD14 CD16 Mo was evaluated. We found that
the entire HUS AP Mo population exhibited reduced
CD14, CD64, and CD11b expression and
decreased LPS-induced tumor necrosis factor production
and Fc-dependent cytotoxicity. HUS AP
showed an increased percentage of CD14
CD16 Mo with higher CD16 and lower CD14
levels compared with the same subset from healthy
children. Moreover, the CD14 CD16 Mo subpopulation
of HUS AP had a decreased HLA-DR
expression, which correlated with severity. In conclusion,
the Mo population from HUS AP patients
presents phenotypic and functional alterations.
The contribution to the pathogenesis and the possible
scenarios that led to these changes are
discussed. RI)/CD64 for phagocytosis and cytotoxicity,
and human leukocyte antigen (HLA)-DR for antigen
presentation. Some of these functions were
also determined. Moreover, the percentage of
CD14 CD16 Mo was evaluated. We found that
the entire HUS AP Mo population exhibited reduced
CD14, CD64, and CD11b expression and
decreased LPS-induced tumor necrosis factor production
and Fc-dependent cytotoxicity. HUS AP
showed an increased percentage of CD14
CD16 Mo with higher CD16 and lower CD14
levels compared with the same subset from healthy
children. Moreover, the CD14 CD16 Mo subpopulation
of HUS AP had a decreased HLA-DR
expression, which correlated with severity. In conclusion,
the Mo population from HUS AP patients
presents phenotypic and functional alterations.
The contribution to the pathogenesis and the possible
scenarios that led to these changes are
discussed. CD16 Mo was evaluated. We found that
the entire HUS AP Mo population exhibited reduced
CD14, CD64, and CD11b expression and
decreased LPS-induced tumor necrosis factor production
and Fc-dependent cytotoxicity. HUS AP
showed an increased percentage of CD14
CD16 Mo with higher CD16 and lower CD14
levels compared with the same subset from healthy
children. Moreover, the CD14 CD16 Mo subpopulation
of HUS AP had a decreased HLA-DR
expression, which correlated with severity. In conclusion,
the Mo population from HUS AP patients
presents phenotypic and functional alterations.
The contribution to the pathogenesis and the possible
scenarios that led to these changes are
discussed. -dependent cytotoxicity. HUS AP
showed an increased percentage of CD14
CD16 Mo with higher CD16 and lower CD14
levels compared with the same subset from healthy
children. Moreover, the CD14 CD16 Mo subpopulation
of HUS AP had a decreased HLA-DR
expression, which correlated with severity. In conclusion,
the Mo population from HUS AP patients
presents phenotypic and functional alterations.
The contribution to the pathogenesis and the possible
scenarios that led to these changes are
discussed. Mo with higher CD16 and lower CD14
levels compared with the same subset from healthy
children. Moreover, the CD14 CD16 Mo subpopulation
of HUS AP had a decreased HLA-DR
expression, which correlated with severity. In conclusion,
the Mo population from HUS AP patients
presents phenotypic and functional alterations.
The contribution to the pathogenesis and the possible
scenarios that led to these changes are
discussed. CD16 Mo subpopulation
of HUS AP had a decreased HLA-DR
expression, which correlated with severity. In conclusion,
the Mo population from HUS AP patients
presents phenotypic and functional alterations.
The contribution to the pathogenesis and the possible
scenarios that led to these changes are
discussed.