Functional analysis of P-bodies components in Drosophila melanogaster.

LAYANA C1,2, FERRERO P1,2, RIVERA POMAR R1,2

Puerto Madryn

Congreso; 46 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2010

Sociedad Argentina de Investigacion en bioquimica y Biologia molecular

  The regulation of mRNA translation involves the remodeling of active ribonucleoprotein complexes (mRNPs) into an inactive state. Part of this process occurs in cytoplasmic granules known as processing bodies (P-bodies), which contain factors required for mRNA turnover and translational repression. The aim of this work is to study the components of P-bodies in Droshopila melanogaster and the interactions involved in mRNP remodelling. We have cloned several components of P-bodies as fluorescent protein fusiones. S2 and HeLa cells were transfected with the constructs and analyzed for P-bodies formation. The proteins Me31b, Lsm1, Bruno and eIF4E were found in P-bodies. In vivo analysis of interactions was studied by fluorescence resonance energy transfer (FRET). We determined the interacting pairs eIF4E and Me31b. RNAi was performed to dilucidated changes in the complex stability to establish a model for sequential remodeling.