Inhalation of marijuana affects Drosophila heart function.

IVANA GÓMEZ; RODRÍGUEZ MAIA; SANTALLA MANUELA; KASISS GEORGE; COLMAN LERNER JE; ARANDA OSWALDO; SEDÁN DANIELA; ANDRINOLO DARÍO; VALVERDE CARLOS A; FERRERO P

BIOLOGY OPEN

Company of Biologists Ltd

Lugar: Cambridge; Año: 2019

2046-6390

We investigated the effect of inhalation of vaporized marijuana on cardiac function in Drosophila melanogaster, a suitable genetic model for studying human diseases. Adult flies were exposed to marijuana for variable time periods and the effects on cardiac function were studied. Short treatment protocol incremented heart rate variability. Contractility was augmented only under prolonged exposure to cannabis and it was associated to incremented calcium transient within cardiomyocytes. Neither the activity of the major proteins responsible for calcium handling nor the calcium load of the sarcoplasmic reticulum were affected by the cannabis treatment. The observed changes manifested in the cardiomyocytes even in absence of the canonical cannabinoid receptors described in mammals. Our results are the first evidence of the in vivo impact of phytocannabinoids in Drosophila melanogaster By providing a simple and affordable platform prior to mammalian models, this characterization of cardiac function under marijuana exposure opens new paths for conducting genetic screenings using vaporized compounds.