Role of phosphorylation of Thr(17) residue of phospholamban in mechanical recovery during hypercapnic acidosis.

MUNDIÑA-WEILENMANN C, FERRERO P, SAID M, VITTONE L, KRANIAS EG, MATTIAZZI A.

CARDIOVASCULAR RESEARCH

Elsevier Science B.V.

Lugar: Amsterdan; Año: 2005 vol. 66 p. 114 - 122

0008-6363

Cardiovasc Res. 2005 Apr 1;66(1):114-22.  Role of phosphorylation of Thr(17) residue of phospholamban in mechanical recovery during hypercapnic acidosis. Mundiña-Weilenmann C, Ferrero P, Said M, Vittone L, Kranias EG, Mattiazzi A. Centro de Investigaciones Cardiovasculares, Facultad de Ciencias Medicas, 60 y 120, (1900) La Plata, Argentina. OBJECTIVES: To assess the time course of phosphorylation of phospholamban residues, the underlying mechanisms determining these phosphorylations, and their functional impact on the mechanical recovery during acidosis. METHODS: Langendorff perfused rat hearts were submitted to 30 min of hypercapnic acidosis. Contractility, relaxation, and phosphorylation of phospholamban residues, immunodetected by specific antibodies, were determined. RESULTS: Acidosis produced a mechanical impairment followed by a spontaneous recovery, most of which occurred within the first 3 min of acidosis (early recovery). During this period, contractility and relaxation recovered by 67+/-9% and 77+/-11%, respectively, from its maximal depression, together with an increase in the Ca(2+)-calmodulin-dependent protein kinase II (CaMKII)-dependent phosphorylation of Thr(17). The CaMKII inhibitor KN-93, at 1, 5 and 10 microM, decreased Thr(17) phosphorylation to basal levels and produced a similar impairment of the early relaxation recovery (50%). However, only 5 and 10 microM KN-93 inhibited the early contractile recovery and completely blunted the late mechanical recovery. Inhibition of the reverse mode of the Na(+)/Ca(2+) exchanger by KB-R7943 decreased Thr(17) phosphorylation but accelerated the early contractile recovery. CONCLUSIONS: CaMKII-dependent Thr(17) phosphorylation significantly increased at the beginning of acidosis, is responsible for 50% of the early relaxation recovery, and is linked to the activation of the reverse Na(+)/Ca(2+) mode. The early contractile recovery and the late mechanical recovery are dependent on CaMKII but independent of the phosphorylation of the Thr(17) residue of phospholamban. The reverse Na(+)/Ca(2+) mode has an additional negative effect that opposes the early mechanical recovery.