INVESTIGADORES
GÜERCI Alba Mabel
artículos
Título:
Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on
Autor/es:
ROSANA CRESPOA, M. LUCIANA VILLAVERDEA, JUAN R. GIROTTI , ALBA GÜERCI, M. PATRICIA JUÁREZA,
Revista:
JOURNAL OF ETHNOPHARMACOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 136 p. 204 - 209
ISSN:
0378-8741
Resumen:
Ethnopharmacological relevance: Ulomoides dermestoides (Fairmaire, 1893) is a cosmopolitan tenebrionid
beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment
of different illnesses such as asthma, Parkinson?s, diabetes, arthritis, HIV and specially cancer.
beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment
of different illnesses such as asthma, Parkinson?s, diabetes, arthritis, HIV and specially cancer.
(Fairmaire, 1893) is a cosmopolitan tenebrionid
beetle reared by Argentine people who consume them alive as an alternative medicine in the treatment
of different illnesses such as asthma, Parkinson?s, diabetes, arthritis, HIV and specially cancer.
Aim of the study: To evaluate the cytotoxicity and DNA damage of the major volatile components released
by Ulomoides dermestoides on human lung carcinoma epithelial cell line A549.
by Ulomoides dermestoides on human lung carcinoma epithelial cell line A549.
To evaluate the cytotoxicity and DNA damage of the major volatile components released
by Ulomoides dermestoides on human lung carcinoma epithelial cell line A549.Ulomoides dermestoides on human lung carcinoma epithelial cell line A549.
Materials and methods: The defence compounds of Ulomoides dermestoides were extracted with
dichloromethane and analyzed and quantified by capillary gas chromatography. The toxicity effects of the
beetle?s extract against A549 cell line were evaluated. Cytotoxicity was evaluated by MTT test and Trypan
blue assay and genotoxicity was evaluated by the comet assay. The synthetic compounds, individually or
combined, were also tested in A549 cells and normal mononuclear human cells.
dichloromethane and analyzed and quantified by capillary gas chromatography. The toxicity effects of the
beetle?s extract against A549 cell line were evaluated. Cytotoxicity was evaluated by MTT test and Trypan
blue assay and genotoxicity was evaluated by the comet assay. The synthetic compounds, individually or
combined, were also tested in A549 cells and normal mononuclear human cells.
The defence compounds of Ulomoides dermestoides were extracted with
dichloromethane and analyzed and quantified by capillary gas chromatography. The toxicity effects of the
beetle?s extract against A549 cell line were evaluated. Cytotoxicity was evaluated by MTT test and Trypan
blue assay and genotoxicity was evaluated by the comet assay. The synthetic compounds, individually or
combined, were also tested in A549 cells and normal mononuclear human cells.
Results: The defence compounds of Ulomoides dermestoides extracted with dichloromethane (methyl-1,4-
benzoquinones, ethyl-1,4-benzoquinones and 1-pentadecene as major components) showed cytotoxic
activity on A549 cells demonstrated by MTT test and Trypan blue assay, with IC50 values of
0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted
per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone
and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect
extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend
(0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2±2.7% and induced significant DNA
damage both in tumor and mononuclear cells.
damage both in tumor and mononuclear cells.
0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted
per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone
and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect
extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend
(0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2±2.7% and induced significant DNA
damage both in tumor and mononuclear cells.
damage both in tumor and mononuclear cells.
benzoquinones, ethyl-1,4-benzoquinones and 1-pentadecene as major components) showed cytotoxic
activity on A549 cells demonstrated by MTT test and Trypan blue assay, with IC50 values of
0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted
per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone
and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect
extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend
(0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2±2.7% and induced significant DNA
damage both in tumor and mononuclear cells.
damage both in tumor and mononuclear cells.
0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted
per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone
and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect
extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend
(0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2±2.7% and induced significant DNA
damage both in tumor and mononuclear cells.
damage both in tumor and mononuclear cells.
The defence compounds of Ulomoides dermestoides extracted with dichloromethane (methyl-1,4-
benzoquinones, ethyl-1,4-benzoquinones and 1-pentadecene as major components) showed cytotoxic
activity on A549 cells demonstrated by MTT test and Trypan blue assay, with IC50 values of
0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted
per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone
and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect
extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend
(0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2±2.7% and induced significant DNA
damage both in tumor and mononuclear cells.
damage both in tumor and mononuclear cells.
0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted
per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone
and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect
extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend
(0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2±2.7% and induced significant DNA
damage both in tumor and mononuclear cells.
damage both in tumor and mononuclear cells.
50 values of
0.26 equivalent/ml and 0.34 equivalent/ml, respectively (1 equivalent = amount of components extracted
per beetle). The inhibition of A549 cell proliferation with the synthetic blend (1,4-benzoquinone
and 1-pentadecene) or 1,4-benzoquinone alone was similar to that obtained with the insect
extract. 1-Pentadecene showed no inhibitory effect. Low doses of insect extract or synthetic blend
(0.15 equivalent/ml) inhibited mononuclear cell proliferation by 72.2±2.7% and induced significant DNA
damage both in tumor and mononuclear cells.
damage both in tumor and mononuclear cells.
±2.7% and induced significant DNA
damage both in tumor and mononuclear cells.
Conclusion: Results of this study demonstrated that defence compounds of Ulomoides dermestoidesResults of this study demonstrated that defence compounds of Ulomoides dermestoides
reduced cell viability and induced DNA damage. We also concluded that the insect benzoquinones are
primarily responsible for inducing cytotoxicity and genotoxicity in culture cells.