Mechanisms involved in the induction of aneuploidy: The significance of chromosome loss”

5- SEOANE ANALÍA I., GÜERCI ALBA M, DULOUT FERNANDO N

GENETICS AND MOLECULAR BIOLOGY

Editada por: Sociedad Brasileira de Genética

Lugar: San Pablo. Brasil.; Año: 2000 vol. 23 p. 1077 - 1082

1415-4757

The induction of aneuploidy  by physical and chemical agents using different test systems was evaluated. The effect of X-rays, caffeine, acetaldehyde, ethanol, diethylstilbestrol  propionaldehyde, and chloral hydrate was studied by chromosome counting in Chinese  hamster embryonic diploid cells. Aneugenic ability of cadmium chloride, cadmium sulfate, potassium dichromate, chromium chloride, nickel chloride, and nickel sulfate was assessed by means of anaphase-telophase analysis in Chinese hamster ovary cells. Chromosome counting in human fibroblasts (MRC-5 cell line) was employed to evaluate the effect of cacodilic acid, cadmium chloride, cadmium sulfate, and potassium dichromate. Finally, the induction of kinetochore-positive and kinetochore negative micronuclei by cadmium  chloride, cadmium sulfate, potassium dichromate, chromium chloride and nickel chloride was studied using CREST antibodies. When the effect of different agents was determined by chromosome counting, an increase of hypoploid but not of hyperploid cells was observed. Anaphase-telophase analysis showed that metal salts increased the frequency of lagging chromosomes. This finding has been confirmed by the increment of kinetochore-positive micronuclei using CREST antibodies. Therefore, chromosome loss could be considered as the main cause of induced aneuploidy.