Biofunctionalization of carbon nanostructures: an interesting alternative for the electrochemical sensing of relevant biomarkers

RIVAS GUSTAVO A.; EGUÍLAZ, MARCOS; GUTIERREZ; RUBIANES, MARIA D.; RODRÍGUEZ MARCELA C.; GALLAY PABLO; LÓPEZ MUJICA MICHAEL, PERRACHIONE FABRIZIO.

Estambul

Congreso; XX Euroanalysis; 2019

The search for novel bioanalytical platforms able to conjugate an efficient immobilization of biomolecules with an improved electroanalytical performance in terms of sensitivity and selectivity,is a key aspect for the development of advanced electrochemical biosensors. In this context, carbon nanostructures have demonstrated to be an excellent material for the development of innovative electrochemical biosensors due to their unique electronic, physical and chemical properties.This presentation will describe new strategies for building biosensing nanoarchitectures based on innovative carbon nanostructures-functionalization schemes using biomolecules that allow the successful exfoliation of the nanostructures and give to them unique properties, either to recognize a given analyte, to work as specific-site for further anchoring of biorecognition molecules, or to facilitate the construction of self-integrated label-free nanobiosensors.The advantages of these new avenues for (bio)markers sensing (glucose, microRNA 21, hydrogen peroxide, galectine3) will be demonstrated in connection with the biofunctionalization of carbon nanomaterials with polypeptides (polyhistidine and polyarginine), biocatalysts (glucose oxidase and cytochrome c), nucleic acis (calf-thymus double stranded DNA), aminoacids (cysteine), and proteins (avidin and concanavalin A) associated with different transduction schemes.The critical selection of the nanostructures-(bio)functionalization strategy and the improved properties of the generated nanohybrid materials, allows the construction of biosensing strategies with enormous potential for the develoment of enzymatic and affinity biosensors and the design of innovative amplificationschemes.