EVALUATION OF LIVER NON-PARENCHYMAL CELLS (NPC) POPULATIONS DURING HIGH FAT DIET (HFD)-DERIVED HEPATIC INFLAMMATORY RESPONSE. ROLE OF TUMOR NECROSIS FACTOR-ALFA RECEPTOR 1 (TNFR1)

MARÍA GUILLERMINA SEDLMEIER; FLAVIA LAMBERTUCCCI; AINELEN ARBOATTI; LUCILA CERE; EDUARDO ROGGERO; GERARDO B. PISANI; JUAN A. MONTI; RONCO, MARIA T.; SILVINA VILLAR; DANIEL ELEAZAR FRANCÉS

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Obesity is a complex metabolic disorder, characterized by chroniclow-grade inflammation, increased pro-inflammatory circulatingcytokines as TNF-a and IL-1β, and immune cell infiltration in theliver. Hepatic non-parenchymal cells, as resident macrophages(Kupffer cells) as well as recruited macrophages, play a significantrole in liver inflammation. In this work, we aim to evaluate the roleof TNFR1 in hepatic inflammation in a model of obesity inducedby HFD. Six-week-old C57BL/6 wild type (WT) and and knockoutC57BL/6-Tnfrsf1atm1Imx/J (TNFR1-KO) mice were fed with regularchow diet (C-WT; C-KO) or a 40% high fat diet for 16 weeks (HFDWT;HFD-KO). Histological evaluation of hepatic tissue stained withhematoxylin and eosin showed mild inflammation in HFD-WT andmoderate to severe inflammation in HFD-KO. To characterize immunecells populations, NPC were isolated from livers by collagenaseperfusion procedure, differential centrifugation and were analyzedanalyzedby flow cytometry. Macrophages content (CD45+F4/80+ cells)was higher in KO mice (HFD-WT:36% vs HFD-KO:51%), in both,resident (CD45+F4/80HIGH cells in HFD-WT:6% vs HFD-KO:10%)and recruited populations (CD45+F4/80LOW cells in HFD-WT:30%vs HFD-KO:40% or CD11b+CCR2+ cells in HFD-WT:2.3% vs HFDKO:9.3%) (p