EFFECT OF Ligaria cuneifolia ( Lc) PROANTHOCYANIDINE ENRICHED FRACTION ON PLASMA LIPIDS LEVELS AND BILE SALTS BILIARY OUTPUT IN WISTAR RATS FED WITH A HIGH FAT DIET (HFD).

FISCH JULIAN ; NATASHA GERSCHCOVSKY; ALICIA DOMINIGHINI; SEBASTIAN GALLIANO; JOSE GONZALVEZ; LEDA URLI; DIEGO CROSETTI; CONSTANZA GIACOSA; JUAN A. MONTI; FLAVIA LAMBERTUCCCI; M. TERESA RONCO; MARCELO LWAGNERC; CARNOVALE CE; ALEJANDRA LUQUITA

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Proanthocyanidin enriched fraction from L.cuneifolia was purified(PL.cuneifolia) to analyze the effect on Cholesterol (Cho) and Triglycerides(TG) plasma levels, and also biliary excretion. Adult maleWistar rats (n = 12), 70 days old, were fed for 28 days with a HFD(every 100g of standard diet 40% bovine meat juice). Rats wereadministered with either physiological solution (controls C, n=6) orPL.cuneifolia 3mg /100g body weight (treated T; n=6) i.p. each 24hrduring 10 days. On day 11 they were anesthetized and the bile ductwas catheterized for bile collection each 15 min for 60 minutes, atthe end, the blood was obtained by cardiac puncture. In plasmaCho, ChoHDL, ChoLDL and TG were determined by enzymatic andcolorimetric methods. Bile flow (BF) was determined by gravimetry,bile salts biliary output (BSO) was calculated (Concentration of bilesalts in bile x BF). Student t test was applied for unpaired data. Resultsare expressed as mean±SD. Cho(mg%): C: 168.00± 6.63, T:100.11±4.91*; ChoHDL: C: 32.20±1.46., T: 28.00 ±2.39 n.s; ChoLDL:C: ChoLDL: C: 24.00±0.63,T: 17.78±0.62*;TG: C: 191.80±21.45, T:133.00±9.68*; BF(μl /min. g liver): C: 2.31±0.049, T: 2.91 ±0.064*;BSO(nmol / min.g liver): C:34.15±3.66, T:54.50±6.50* (*p