TYPE 2 DIABETES MELLITUS (DM2) INDUCED BY HIGH-FAT DIET (HFD) IN MICE ARE SENSITIZED TO DEN-INDUCED HEPATIC CARCINOGENESIS

AINELEN ARBOATTI; FLAVIA LAMBERTUCCCI; MARÍA GUILLERMINA SEDLMEIER; GERARDO B. PISANI; MONTI JUAN A; DANIEL ELEAZAR FRANCÉS; RONCO MARIA TERESA; CARNOVALE CRISTINA E

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2016

ia,insulin resistance, and relative insulin deficiency. DM2 resultsfrom interaction between genetic, environmental and behavioral riskfactors. Epidemiological studies indicate that DM2 is associated withincreased risk of developing cancer. We study the liver sensitivity ofDM2 (induced by HFD) to development of hepatocellular carcinoma(HCC) Therefore we have used the carcinogenic agent diethylnitrosamine(DEN), analyzing the proliferative process as early stageof hepatic carcinogenesis. Five-week-old mice C57BL/6 were randomlydivided into 4 experimental groups: Control (C, mice werefed a normal chow diet), C treated with DEN (C+DEN), HFD micethat fed a high-fat diet and HFD+DEN animals that fed a high-fatdiet and received DEN at 16 weeks (n=4 in each group). Mice wereeuthanized at 25 weeks after DEN-injection. In liver sections we performedhistological studies and quantification of hepatocytes in thedifferent phases of the cell cycle (immunohistochemistry of PCNA).HFD+DEN showed an increase in hepatocytes in phases G1, S andM as well as proliferative index (PI) compared to C (p