This evidences point to a novel functional role for TNFR1 in the pathogenesis of NAFLD.

NATASHA GERSCHCOVSKY; SEBASTIAN GALLIAN; GLORIA GARCÍA1,; ALICIA DOMINIGHINI; JOSÉ GONZÁLVEZ; LEDA URLI; DIEGO CROSETTI; JUAN MONTI; FLAVIA LAMBERTUCCI; Y MARÍA TERESA RONCO.; MARCELO WAGNER;; CRISTINA ESTER CARNOVALE; ALEJANDRA NORA LUQUITA

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC) LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) XLVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE) VII REUNIÓN ANUAL DE LA SOCIEDAD; 2016

We have previously demonstrated that intraperitoneal (i.p.)injection of Lc crude extract produces a plasma cholesterol (Cho)decrease and blood viscosity increase in rats. Proanthocyanidine(PLc) was purified to analyze the effect on Cho, Triglyceridesand blood fluidity in adult male Wistar rats (aged 70 days, n=16)fed with standard chow diet added with 40% bovine meat juiceduring 28 days (HFD). Rats were administered with either physiologicalsolution (controls C, n=8) or PLc 3mg /100g body weight(treated T; n=8) i.p. each 24hr during 7 days. On day 8 theywere anesthetized i.p. with Ketamine/Xylazine (100mg/kg/3mg/kg) to obtain blood samples by cardiac puncture. Plasma assays:Cho (enzymatic method with cholesterol oxidase esterase),Triglycerides, HDL-Cho, and LDL-Cho. Blood assays: total bloodviscosity (TBV) and plasma viscosity (PV) by rotational viscosimeter.Standardized relative BV (SBV) to a 45% hematocrit [(BV/PV)45/Hto]; rigidity index (RI), the inverse of erythrocyte deformability(ED) by filtration method. Results: (mean ± SD). Plasma Cho(mg%): C:145.25±8.53 , T: 62.88±3.23**; ChoHDL: C:25.00±1.35;T:29.5±2.29 (n.s.); ChoLDL: C:28.86±2.41; T:21.37±2.19*; Triglycerides:C:360.71±27.48, T:71.38±11.04**; SBV to a 45% hematocrit:C:5.09±0.45, T:5.41±0.49(n.s.); RI: C:8.37±1.45, T:9.61±1.43(n.s.);Mean Corpuscular Volume (μm3): C:72.02±5.74; T:65.26±4.65(n.s.)(*p