Nitric oxide (NO) regulation of C/EBP-α and Ref-1 in early stage of post-partial hepatectomy in streptozotocin (SZ)-induced diabetic rats

INGARAMO P; RONCO M.T; FRANCES D.E; MONTI J.A; CEBALLOS MP; CARRILLO, M.C; CARNOVALE CE

Congreso; VII Meeting of SFRBM South American Group, Free Radicals and Antioxidants; 2011

It is known that a decrease in liver regeneration exists in diabetes state. In previous works we showed that NO increased after partial hepatectomy (PH). We also demonstrated that it is augmented in diabetic state. The transcriptional factor C/EBPα may have an anti-proliferative function. Ref-1 is a protein that can regulate the redox state of diverse proteins and functions as a DNA repair endonuclease. Aim: To evaluate the NO regulation of C/EBPα and Ref-1 after 1 hour post-PH in liver of diabetic rats. Methodology: Adult male Wistar rats were randomized into 2 groups: control (C, citrate buffer pH=4.5, i.p.) and diabetic (D, SZ, 60 mg / kg of body weight, i.p.). Thirty days post-injection of either citrate or SZ, 10 rats of each group were subjected to PH of 70 % and were evaluated 1 h post-surgery. Five rats of each group were daily treated with 100 mg/kg body weight of aminoguanidine (AG), a selective inhibitor, on the 3 days previous to surgery (PH-C+AG and PH-D+AG). Results: Glycemia (enzymatic method, g/l): PH-C: 6,4±0,3; PH-D: 25,7±3,1*; Body weight (g): PH-C: 438±7; PH-D: 296±6*; (*p