EFFECT OF LIGARIA CUNEIFOLIA (Lc) - PROTOANTHOCYANIDINE ENRICHED FRACTION ON HEPATIC HISTOLOGY IN HIGH FAT DIET (HFD) FED RATS

JUAN ALONSO; CAROLINA GALIASSO; SEBASTIAN GALEANO; DIEGO CROSETTI; LEDA URLI; ROMINA SERENA; DANIEL ELEAZAR FRANCÉS; MARÍA TERESA RONCO; MARCELO LWAGNERC; CARNOVALE, CRISTINA; ALEJANDRA LUQUITA;

Congreso; reunion biociencias 2022; 2022

Previously we demonstrated that fraction enriched in Proanthocyanidin obtained from Ligaria cuneifolia (PLc) decreased blood viscosityby lowering cholesterol (Co) and plasma triglycerides levels (TG)in rats fed with a high-fat diet (HFD). However, the effect of PLctreatment on liver histology after a HFD had not been studied. Theaim of this work was to evaluate the effect of PLc treatment during7 and 10 days using NASH score and PAS histochemical assay todeterminate glucogen content in liver of HFD feds rats. Adult maleWistar rats (aged 70 days, n=18) were fed with standard chow dietadded with 40% bovine meat juice during 28 days (HFD) and wereadministered i.p. each 24hr during 7 and 10 days with either salinevehicle (HFD) or PLc 3mg /100g b.w. (HFD-PLc7 and HFD-PLc10).At the end of PLc-treatment, animals were anesthetized i.p. withKetamine/Xylazine (100mg/kg/3mg/kg), blood samples were obtained by cardiac puncture and samples of liver tissue were fixed in4% buffered formalin for paraffin preparation for Heamtoxilin&eosine(H&E) tinction. Another set of samples was processed for PAS (Periodic Acid-Schiff) histochemistry. To assess the diet-induced model,plasmatic glucose levels were determined (Accu-Check® Glucometer, Roche) Results are expressed as medium ± SEM. Glu (mg/dl): HFD: 264.6 ± 16.02, PLc7: 134± 4.03*, PLc10: 131.6± 2.80*(*p